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ATCC ovcar 8
Ovcar 8, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 7540 article reviews

ovcar 8 - by Bioz Stars, 2026-05

99/100 stars

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ovcar 8 (ATCC)

ATCC ovcar 8
Ovcar 8, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ovcar 8/product/ATCC
Average 99 stars, based on 1 article reviews

ovcar 8 - by Bioz Stars, 2026-05

99/100 stars

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ovcar8 (CLS Cell Lines Service GmbH)

CLS Cell Lines Service GmbH ovcar8
$a Schematic representation of adipose tissue-mimicking collagen-based organo-hydrogels (OHGs). b BODIPY (green) staining of human peritoneal adipose tissue and OHG (representative images from n = 7 patients and n = 3 OHGs). c Quantification of human peritoneal adipocyte ( n = 100 adipocytes/tissue from 7 patients) and silicone oil microdroplet ( n = 100 microdroplets from 3 OHGs) diameter. d Quantification of volume fraction occupied by oil in peritoneal adipose tissues ( n = 7 patients) and OHGs ( n = 3 gels). e Storage moduli of hydrogels and human peritoneal tissues ( n = 5 gels; n = 9 adipose and n = 5 connective tissues). f Normalised stress-relaxation curves of hydrogels and human peritoneal tissues ( n = 5 gels or tissues). g Spheroid area after 7 d relative to day 0 of multiple ovarian cancer cell lines ( n = 3 spheroids). h Spheroid area comparison of ovarian cancer cell lines after 7 d in collagen or OHGs relative to the collagen average ( n = 3 spheroids). i Collagen-I (grey) and <t>mRFP-OVCAR8</t> nuclei (red) staining of OVCAR8 cells seeded on top of collagen or OHG at 25 μm gel depth after 7 d in culture (representative images from n = 3 experiments). j Quantification of hydrogel organotypic invasion of OVCAR8 cells after 7 d ( n = 3 experiments). Rhombuses indicate the average. k BODIPY (green), mRFP-OVCAR8 (red), and collagen I (grey) staining of peritoneal tissue explants and mRFP-OVCAR8 cells after 7 d in culture (representative images from n = 3 experiments). Arrowheads indicate invading mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells in peritoneal tissue explants and OHGs (42 μm depth) after 7 d culture (representative images from n = 3 experiments). n Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues or OHGs after 7 d ( n = 3 tissues from distinct donors or gels). Rhombuses indicate the average. For the data in ( c , d , g , h , j , l and n ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( e ). Error bars in ( e ) represent the s.e.m. Scale bars, 50 μm ( b , i , m ), 100 μm ( k ). Components of ( a ) have been created in BioRender. Gautrot, J. (2025) https://BioRender.com/rk3jchz . Source data are provided as a Source Data file.$
Ovcar8, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ovcar8/product/CLS Cell Lines Service GmbH
Average 94 stars, based on 1 article reviews

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ovcar 8 cells (Creative Biolabs)

Creative Biolabs ovcar 8 cells
$a Schematic representation of adipose tissue-mimicking collagen-based organo-hydrogels (OHGs). b BODIPY (green) staining of human peritoneal adipose tissue and OHG (representative images from n = 7 patients and n = 3 OHGs). c Quantification of human peritoneal adipocyte ( n = 100 adipocytes/tissue from 7 patients) and silicone oil microdroplet ( n = 100 microdroplets from 3 OHGs) diameter. d Quantification of volume fraction occupied by oil in peritoneal adipose tissues ( n = 7 patients) and OHGs ( n = 3 gels). e Storage moduli of hydrogels and human peritoneal tissues ( n = 5 gels; n = 9 adipose and n = 5 connective tissues). f Normalised stress-relaxation curves of hydrogels and human peritoneal tissues ( n = 5 gels or tissues). g Spheroid area after 7 d relative to day 0 of multiple ovarian cancer cell lines ( n = 3 spheroids). h Spheroid area comparison of ovarian cancer cell lines after 7 d in collagen or OHGs relative to the collagen average ( n = 3 spheroids). i Collagen-I (grey) and <t>mRFP-OVCAR8</t> nuclei (red) staining of OVCAR8 cells seeded on top of collagen or OHG at 25 μm gel depth after 7 d in culture (representative images from n = 3 experiments). j Quantification of hydrogel organotypic invasion of OVCAR8 cells after 7 d ( n = 3 experiments). Rhombuses indicate the average. k BODIPY (green), mRFP-OVCAR8 (red), and collagen I (grey) staining of peritoneal tissue explants and mRFP-OVCAR8 cells after 7 d in culture (representative images from n = 3 experiments). Arrowheads indicate invading mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells in peritoneal tissue explants and OHGs (42 μm depth) after 7 d culture (representative images from n = 3 experiments). n Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues or OHGs after 7 d ( n = 3 tissues from distinct donors or gels). Rhombuses indicate the average. For the data in ( c , d , g , h , j , l and n ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( e ). Error bars in ( e ) represent the s.e.m. Scale bars, 50 μm ( b , i , m ), 100 μm ( k ). Components of ( a ) have been created in BioRender. Gautrot, J. (2025) https://BioRender.com/rk3jchz . Source data are provided as a Source Data file.$
Ovcar 8 Cells, supplied by Creative Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews

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ht29 21 ovcar (ATCC)

ATCC ht29 21 ovcar
$a Schematic representation of adipose tissue-mimicking collagen-based organo-hydrogels (OHGs). b BODIPY (green) staining of human peritoneal adipose tissue and OHG (representative images from n = 7 patients and n = 3 OHGs). c Quantification of human peritoneal adipocyte ( n = 100 adipocytes/tissue from 7 patients) and silicone oil microdroplet ( n = 100 microdroplets from 3 OHGs) diameter. d Quantification of volume fraction occupied by oil in peritoneal adipose tissues ( n = 7 patients) and OHGs ( n = 3 gels). e Storage moduli of hydrogels and human peritoneal tissues ( n = 5 gels; n = 9 adipose and n = 5 connective tissues). f Normalised stress-relaxation curves of hydrogels and human peritoneal tissues ( n = 5 gels or tissues). g Spheroid area after 7 d relative to day 0 of multiple ovarian cancer cell lines ( n = 3 spheroids). h Spheroid area comparison of ovarian cancer cell lines after 7 d in collagen or OHGs relative to the collagen average ( n = 3 spheroids). i Collagen-I (grey) and <t>mRFP-OVCAR8</t> nuclei (red) staining of OVCAR8 cells seeded on top of collagen or OHG at 25 μm gel depth after 7 d in culture (representative images from n = 3 experiments). j Quantification of hydrogel organotypic invasion of OVCAR8 cells after 7 d ( n = 3 experiments). Rhombuses indicate the average. k BODIPY (green), mRFP-OVCAR8 (red), and collagen I (grey) staining of peritoneal tissue explants and mRFP-OVCAR8 cells after 7 d in culture (representative images from n = 3 experiments). Arrowheads indicate invading mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells in peritoneal tissue explants and OHGs (42 μm depth) after 7 d culture (representative images from n = 3 experiments). n Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues or OHGs after 7 d ( n = 3 tissues from distinct donors or gels). Rhombuses indicate the average. For the data in ( c , d , g , h , j , l and n ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( e ). Error bars in ( e ) represent the s.e.m. Scale bars, 50 μm ( b , i , m ), 100 μm ( k ). Components of ( a ) have been created in BioRender. Gautrot, J. (2025) https://BioRender.com/rk3jchz . Source data are provided as a Source Data file.$
Ht29 21 Ovcar, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ht29 21 ovcar/product/ATCC
Average 99 stars, based on 1 article reviews

ht29 21 ovcar - by Bioz Stars, 2026-05

99/100 stars

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$a Schematic representation of adipose tissue-mimicking collagen-based organo-hydrogels (OHGs). b BODIPY (green) staining of human peritoneal adipose tissue and OHG (representative images from n = 7 patients and n = 3 OHGs). c Quantification of human peritoneal adipocyte ( n = 100 adipocytes/tissue from 7 patients) and silicone oil microdroplet ( n = 100 microdroplets from 3 OHGs) diameter. d Quantification of volume fraction occupied by oil in peritoneal adipose tissues ( n = 7 patients) and OHGs ( n = 3 gels). e Storage moduli of hydrogels and human peritoneal tissues ( n = 5 gels; n = 9 adipose and n = 5 connective tissues). f Normalised stress-relaxation curves of hydrogels and human peritoneal tissues ( n = 5 gels or tissues). g Spheroid area after 7 d relative to day 0 of multiple ovarian cancer cell lines ( n = 3 spheroids). h Spheroid area comparison of ovarian cancer cell lines after 7 d in collagen or OHGs relative to the collagen average ( n = 3 spheroids). i Collagen-I (grey) and mRFP-OVCAR8 nuclei (red) staining of OVCAR8 cells seeded on top of collagen or OHG at 25 μm gel depth after 7 d in culture (representative images from n = 3 experiments). j Quantification of hydrogel organotypic invasion of OVCAR8 cells after 7 d ( n = 3 experiments). Rhombuses indicate the average. k BODIPY (green), mRFP-OVCAR8 (red), and collagen I (grey) staining of peritoneal tissue explants and mRFP-OVCAR8 cells after 7 d in culture (representative images from n = 3 experiments). Arrowheads indicate invading mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells in peritoneal tissue explants and OHGs (42 μm depth) after 7 d culture (representative images from n = 3 experiments). n Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues or OHGs after 7 d ( n = 3 tissues from distinct donors or gels). Rhombuses indicate the average. For the data in ( c , d , g , h , j , l and n ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( e ). Error bars in ( e ) represent the s.e.m. Scale bars, 50 μm ( b , i , m ), 100 μm ( k ). Components of ( a ) have been created in BioRender. Gautrot, J. (2025) https://BioRender.com/rk3jchz . Source data are provided as a Source Data file.$

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a Schematic representation of adipose tissue-mimicking collagen-based organo-hydrogels (OHGs). b BODIPY (green) staining of human peritoneal adipose tissue and OHG (representative images from n = 7 patients and n = 3 OHGs). c Quantification of human peritoneal adipocyte ( n = 100 adipocytes/tissue from 7 patients) and silicone oil microdroplet ( n = 100 microdroplets from 3 OHGs) diameter. d Quantification of volume fraction occupied by oil in peritoneal adipose tissues ( n = 7 patients) and OHGs ( n = 3 gels). e Storage moduli of hydrogels and human peritoneal tissues ( n = 5 gels; n = 9 adipose and n = 5 connective tissues). f Normalised stress-relaxation curves of hydrogels and human peritoneal tissues ( n = 5 gels or tissues). g Spheroid area after 7 d relative to day 0 of multiple ovarian cancer cell lines ( n = 3 spheroids). h Spheroid area comparison of ovarian cancer cell lines after 7 d in collagen or OHGs relative to the collagen average ( n = 3 spheroids). i Collagen-I (grey) and mRFP-OVCAR8 nuclei (red) staining of OVCAR8 cells seeded on top of collagen or OHG at 25 μm gel depth after 7 d in culture (representative images from n = 3 experiments). j Quantification of hydrogel organotypic invasion of OVCAR8 cells after 7 d ( n = 3 experiments). Rhombuses indicate the average. k BODIPY (green), mRFP-OVCAR8 (red), and collagen I (grey) staining of peritoneal tissue explants and mRFP-OVCAR8 cells after 7 d in culture (representative images from n = 3 experiments). Arrowheads indicate invading mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells in peritoneal tissue explants and OHGs (42 μm depth) after 7 d culture (representative images from n = 3 experiments). n Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues or OHGs after 7 d ( n = 3 tissues from distinct donors or gels). Rhombuses indicate the average. For the data in ( c , d , g , h , j , l and n ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( e ). Error bars in ( e ) represent the s.e.m. Scale bars, 50 μm ( b , i , m ), 100 μm ( k ). Components of ( a ) have been created in BioRender. Gautrot, J. (2025) https://BioRender.com/rk3jchz . Source data are provided as a Source Data file.

Article Snippet: OVCAR3 (HTB-161, ATCC), OVCAR8 (305383, Cytion), OVCAR4 (SCC258, Sigma), Kuramochi (JCRB0098, JCRB) and CAOV3 (HTB-75, ATCC) cells were grown in RPMI medium, while Tyk-nu (JCRB0234.0, JCRB) and Tyk-nu.CPR (JCRB0234.1, JCRB) were cultured in Minimum Essential Medium (MEM).

Techniques: Staining, Comparison

a pFAK, pMLC, YAP/TAZ (green; left to right) and nuclei (blue) staining of OVCAR8 cells in collagen or organo-hydrogel (OHG) after 48 h in culture (representative images from n = 3 gels). b Quantification of immunofluorescence signal intensity of cytoplasmic pFAK, pMLC, relative to the average in collagen and cytoplasmic:nuclear ratio of YAP/TAZ in OVCAR8 cells embedded in collagen or OHG for 48 h ( n = 24 cells). c F-actin (yellow), nucleus (blue) and BODIPY (grey) staining of an OVCAR8 cell spread at the ECM-microdroplet interface. d Shape descriptors of OVCAR8 nuclei after 24 h in collagen or OHG ( n = 53 nuclei). e Relative spheroid area of OVCAR8 after 7 d in OHG ( n = 10 spheroids). f BODIPY (green) and mRFP (red) staining of peritoneal adipose tissue explants and mRFP-OVCAR8 cells (49 μm into the tissue from the surface) after 7 d in culture (representative images from n = 3 experiments). g Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. h Nuclei (red) of OVCAR8 cells in OHGs after 7 d treatment (representative images from n = 14 spheroids). i Quantification of spheroid area relative to DMSO control of OVCAR8 cells in OHGs ( n = 14 spheroids). i F-actin (green) and nuclei staining of CAOV3 spheroids embedded in OHGs for 7 d (representative images from n = 6 spheroids). k Quantification of spheroid area in CAOV3 cells in OHGs after 7 d ( n = 6 spheroids). l F-actin (green) and nuclei (blue) staining of Kuramochi spheroids in collagen or OHG for 7 d with or without TGFβ (representative images from n = 3 spheroids). For the data in ( b , d , g , i and k ), a two-sided unpaired t test was performed. For data in ( e ), a one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed. Scale bars, 25 μm ( a , c ), 100 μm ( f , I ), 200 μm ( h , j ). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a pFAK, pMLC, YAP/TAZ (green; left to right) and nuclei (blue) staining of OVCAR8 cells in collagen or organo-hydrogel (OHG) after 48 h in culture (representative images from n = 3 gels). b Quantification of immunofluorescence signal intensity of cytoplasmic pFAK, pMLC, relative to the average in collagen and cytoplasmic:nuclear ratio of YAP/TAZ in OVCAR8 cells embedded in collagen or OHG for 48 h ( n = 24 cells). c F-actin (yellow), nucleus (blue) and BODIPY (grey) staining of an OVCAR8 cell spread at the ECM-microdroplet interface. d Shape descriptors of OVCAR8 nuclei after 24 h in collagen or OHG ( n = 53 nuclei). e Relative spheroid area of OVCAR8 after 7 d in OHG ( n = 10 spheroids). f BODIPY (green) and mRFP (red) staining of peritoneal adipose tissue explants and mRFP-OVCAR8 cells (49 μm into the tissue from the surface) after 7 d in culture (representative images from n = 3 experiments). g Quantification of mRFP-OVCAR8 organotypic invasion into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. h Nuclei (red) of OVCAR8 cells in OHGs after 7 d treatment (representative images from n = 14 spheroids). i Quantification of spheroid area relative to DMSO control of OVCAR8 cells in OHGs ( n = 14 spheroids). i F-actin (green) and nuclei staining of CAOV3 spheroids embedded in OHGs for 7 d (representative images from n = 6 spheroids). k Quantification of spheroid area in CAOV3 cells in OHGs after 7 d ( n = 6 spheroids). l F-actin (green) and nuclei (blue) staining of Kuramochi spheroids in collagen or OHG for 7 d with or without TGFβ (representative images from n = 3 spheroids). For the data in ( b , d , g , i and k ), a two-sided unpaired t test was performed. For data in ( e ), a one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed. Scale bars, 25 μm ( a , c ), 100 μm ( f , I ), 200 μm ( h , j ). Source data are provided as a Source Data file.

Techniques: Staining, Immunofluorescence, Control

a Schematic representation of norbornene-functionalised hyaluronic acid (NB-HA)-based organohydrogels (OHGs) presenting matrix metalloproteinase (MMP)-degradable crosslinking peptides and cell adhesion ligands. b Storage moduli of collagen- and NB-HA-based OHGs ( n = 5 collagen-based and n = 3 NB-HA-based OHGs; average ± s.e.m.). c Normalised stress-relaxation curves of collagen- and HA-NB OHG ( n = 5 collagen-based and n = 3 NB-HA-based OHGs). d F-actin (grey) and nuclei (red) in OVCAR8 cells embedded in collagen- or NB-HA-based OHGs for 7 d (representative images from n = 9 spheroids). e OVCAR8 spheroid area in collagen- or NB-HA-based OHGs relative to collagen-based OHG average ( n = 9 spheroids). f F-actin (grey) and nuclei (red) of OVCAR8 cells embedded in GFOGER- or RGD-presenting NB-HA OHG (representative images from n = 9 spheroids). g BODIPY (green), nuclei (red), and F-actin (grey) of OVCAR8 in GFOGER- or RGD-presenting NB-HA OHG (representative images from n = 9 spheroids). Arrowheads indicate cells in direct contact with oil microdroplets. h OVCAR8 spheroid area in GFOGER- or RGD-presenting NB-HA OHG after 7 d culture relative to GFOGER OHG average ( n = 9 spheroids). i F-actin (grey) and nuclei (red) in OVCAR8 cells embedded in collagen-based OHGs for 7 d (representative images from n = 8 spheroids). j OVCAR8 spheroid area in collagen-based OHGs after 7 d culture relative to DMSO control ( n = 8 spheroids). k BODIPY (green) and mRFP (red) staining of peritoneal adipose tissue explants and mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion depth into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m OVCAR8 spheroid area quantifications in MMP-cleavable or non-cleavable NB-HA OHG after 7 d culture relative to MMP-cleavable control ( n = 11 spheroids). For the data in ( b , e , h , j and l ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( m ). Error bars in ( b ) represent the s.e.m. Scale bars, 100 μm ( g , k ), 200 μm ( d , f , i ). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a Schematic representation of norbornene-functionalised hyaluronic acid (NB-HA)-based organohydrogels (OHGs) presenting matrix metalloproteinase (MMP)-degradable crosslinking peptides and cell adhesion ligands. b Storage moduli of collagen- and NB-HA-based OHGs ( n = 5 collagen-based and n = 3 NB-HA-based OHGs; average ± s.e.m.). c Normalised stress-relaxation curves of collagen- and HA-NB OHG ( n = 5 collagen-based and n = 3 NB-HA-based OHGs). d F-actin (grey) and nuclei (red) in OVCAR8 cells embedded in collagen- or NB-HA-based OHGs for 7 d (representative images from n = 9 spheroids). e OVCAR8 spheroid area in collagen- or NB-HA-based OHGs relative to collagen-based OHG average ( n = 9 spheroids). f F-actin (grey) and nuclei (red) of OVCAR8 cells embedded in GFOGER- or RGD-presenting NB-HA OHG (representative images from n = 9 spheroids). g BODIPY (green), nuclei (red), and F-actin (grey) of OVCAR8 in GFOGER- or RGD-presenting NB-HA OHG (representative images from n = 9 spheroids). Arrowheads indicate cells in direct contact with oil microdroplets. h OVCAR8 spheroid area in GFOGER- or RGD-presenting NB-HA OHG after 7 d culture relative to GFOGER OHG average ( n = 9 spheroids). i F-actin (grey) and nuclei (red) in OVCAR8 cells embedded in collagen-based OHGs for 7 d (representative images from n = 8 spheroids). j OVCAR8 spheroid area in collagen-based OHGs after 7 d culture relative to DMSO control ( n = 8 spheroids). k BODIPY (green) and mRFP (red) staining of peritoneal adipose tissue explants and mRFP-OVCAR8 cells. l Quantification of mRFP-OVCAR8 organotypic invasion depth into human peritoneal tissues after 7 d ( n = 3 tissues from distinct donors). Rhombuses indicate the average. m OVCAR8 spheroid area quantifications in MMP-cleavable or non-cleavable NB-HA OHG after 7 d culture relative to MMP-cleavable control ( n = 11 spheroids). For the data in ( b , e , h , j and l ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( m ). Error bars in ( b ) represent the s.e.m. Scale bars, 100 μm ( g , k ), 200 μm ( d , f , i ). Source data are provided as a Source Data file.

Techniques: Control, Staining

a F-actin (grey) and DAPI (blue) in OVCAR8 cells embedded in collagen or collagen-based organo-hydrogels (OHG) for 24 h (representative images from n = 3 gels). Microdroplet locations are indicated with an asterisk. b BODIPY (green), F-actin (grey) and nuclei (blue) staining of OVCAR8 cells embedded in collagen-based OHG (left; n = 3 gels) or BODIPY (green), mRFP (red) staining of mRFP-OVCAR8 cells invading into peritoneal adipose tissue (right; n = 3 tissues from 1 donor). Arrowheads indicate oil microdroplet (left) and adipocyte (right) deformations at the contact points with cells. c Quantification of OVCAR8 organotypic invasion depth into peritoneal adipose tissues after 7 d ( n = 3 tissues from 1 donor). Rhombuses indicate the average. d Schematic representation of the Sylgard 184 PDMS-based OGH. e F-actin (grey) and nuclei (red) staining of OVCAR8 spheroids embedded for 7 d in collagen-based OHGs prepared with PDMS microdroplets of varying stiffness (representative images from n = 10 spheroids). f OVCAR8 spheroid area after 7 d in collagen-based OHGs with PDMS microdroplets prepared with varying crosslinker percentage, relative to average area in 2 wt% microbead OHGs ( n = 10 spheroids). g Schematic representation of the norbornene-functionalised hyaluronic acid (NB-HA) OGH with norbornene-functionalised bovine serum albumin (NB-BSA) used for RGD presentation at the microdroplet surface. h F-actin (grey) and nuclei (red) of OVCAR8 spheroids after 7 d in NB-HA OHG with localised presentation of RGD (left). Relative OVCAR8 spheroid area in NB-HA OHG with localised RGD presentation after 7 d in culture ( n = 11 spheroids; top right). BODIPY (green), nuclei (red), and F-actin (grey) staining of OVCAR8 cells in NB-HA OHG presenting RGD on the microdroplet surface (bottom right). Arrowhead indicates a cell spreading at the microdroplet-HA interface. i Schematic representation of the system to modulate microdroplet interfacial mechanics. j F-actin (grey) and nuclei (red) of OVCAR8 spheroids after 7 d in NB-HA OHGs (microdroplet-restricted RGD presentation) with varying microdroplet interfacial modulus (representative images from n = 9 spheroids). k OVCAR8 spheroid area in NB-HA OHGs with microdroplet-presenting RGD and varying protein nanosheet interfacial mechanics after 7 d in culture, relative to BSA-only control ( n = 9 spheroids). For the data in ( c and h ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( f and k ). Scale bars, 25 μm ( a , b ), 50 μm ( h , bottom right), 200 μm ( e , h left, j ). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a F-actin (grey) and DAPI (blue) in OVCAR8 cells embedded in collagen or collagen-based organo-hydrogels (OHG) for 24 h (representative images from n = 3 gels). Microdroplet locations are indicated with an asterisk. b BODIPY (green), F-actin (grey) and nuclei (blue) staining of OVCAR8 cells embedded in collagen-based OHG (left; n = 3 gels) or BODIPY (green), mRFP (red) staining of mRFP-OVCAR8 cells invading into peritoneal adipose tissue (right; n = 3 tissues from 1 donor). Arrowheads indicate oil microdroplet (left) and adipocyte (right) deformations at the contact points with cells. c Quantification of OVCAR8 organotypic invasion depth into peritoneal adipose tissues after 7 d ( n = 3 tissues from 1 donor). Rhombuses indicate the average. d Schematic representation of the Sylgard 184 PDMS-based OGH. e F-actin (grey) and nuclei (red) staining of OVCAR8 spheroids embedded for 7 d in collagen-based OHGs prepared with PDMS microdroplets of varying stiffness (representative images from n = 10 spheroids). f OVCAR8 spheroid area after 7 d in collagen-based OHGs with PDMS microdroplets prepared with varying crosslinker percentage, relative to average area in 2 wt% microbead OHGs ( n = 10 spheroids). g Schematic representation of the norbornene-functionalised hyaluronic acid (NB-HA) OGH with norbornene-functionalised bovine serum albumin (NB-BSA) used for RGD presentation at the microdroplet surface. h F-actin (grey) and nuclei (red) of OVCAR8 spheroids after 7 d in NB-HA OHG with localised presentation of RGD (left). Relative OVCAR8 spheroid area in NB-HA OHG with localised RGD presentation after 7 d in culture ( n = 11 spheroids; top right). BODIPY (green), nuclei (red), and F-actin (grey) staining of OVCAR8 cells in NB-HA OHG presenting RGD on the microdroplet surface (bottom right). Arrowhead indicates a cell spreading at the microdroplet-HA interface. i Schematic representation of the system to modulate microdroplet interfacial mechanics. j F-actin (grey) and nuclei (red) of OVCAR8 spheroids after 7 d in NB-HA OHGs (microdroplet-restricted RGD presentation) with varying microdroplet interfacial modulus (representative images from n = 9 spheroids). k OVCAR8 spheroid area in NB-HA OHGs with microdroplet-presenting RGD and varying protein nanosheet interfacial mechanics after 7 d in culture, relative to BSA-only control ( n = 9 spheroids). For the data in ( c and h ), a two-sided unpaired t test was performed. One-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons was performed for the data in ( f and k ). Scale bars, 25 μm ( a , b ), 50 μm ( h , bottom right), 200 μm ( e , h left, j ). Source data are provided as a Source Data file.

Techniques: Staining, Control

$a Schematic representation of the organo-hydrogels (OHGs) with varying microdroplet diameter and volume fraction. b Storage modulus of collagen-based OHG with distinct microdroplet size ( n = 4 gels; average ± s.e.m.). The dashed line shows the predicted trend. c Normalised stress-relaxation curves of collagen-based OHG with distinct microdroplet size ( n = 4 gels). d BODIPY (green) and F-actin (red) and nuclei (blue) staining of OVCAR8 cells after 7 d in collagen-based OHG of 70 or 25 μm diameter microdroplets (representative images from n = 13 spheroids). Arrowheads indicate cells at the invasive front. e Quantification of relative OVCAR8 spheroid area in collagen-based OHG of distinct emulsion percentage of volume fraction and microdroplet diameter after 7 d in culture ( n = 13 spheroids). f BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells after 7 d invasion into adipose peritoneal tissue explants (representative images from n = 3 explants per donor). g Correlation between patient average adipocyte diameter and invaded OVCAR8 cells after 7 d, at 42 μm tissue depth, relative to number of cells at the tissue surface ( n = 6 donors; average ± s.e.m.). The dashed line shows the predicted trend. h Quantification of interdroplet distance in OHG ( n = 100 microdroplets). i Quantification of the percentage of invasive front OVCAR8 cells in contact with microdroplets in OHG ( n = 121 cells in 3 gels). j Quantification of percentage of Ki67 + cells, immunofluorescence signal intensity of cytoplasmic pFAK and pMLC relative to collagen average, and nuclear:cytoplasmic ratio of YAP/TAZ in OVCAR8 cells embedded in OHG of 70 or 25 μm diameter microdroplets for 24 h ( n = 55 cells). k F-actin (green) and DAPI (blue) staining of CAOV3 spheroids embedded for 7 d in collagen-based 25 μm microdroplet OHG in the presence or absence of TGFβ. Arrowheads indicate invading cells (representative images from n = 6 spheroids). l Quantification of CAOV3 spheroid area relative to non-TGFβ-treated control after 7 d in culture ( n = 6 spheroids). m Schematic illustration of the cell force-dependent invasion of adipose tissue enabled by the anisotropic mechanics of OHGs and the generation of migration tracks at the ECM-adipocyte/microdroplet interface. Arrows indicate the direction of the force. For the data in ( h , i , j and l ), a two-sided unpaired t test was performed. Coefficient of determination and Pearson correlation (two-tailed test) were performed in ( g ) to determine the relationship between tissue adipocyte diameter and OVCAR8 invasion. Error bars in ( b ) represent the s.e.m. Scale bars, 50 μm (bottom panels in d , k ), 100 μm ( f ), 200 μm (top panels in d , k ). Source data are provided as a Source Data file.$

Journal: Nature Communications

Article Title: Biomimetic organo-hydrogels reveal the adipose tissue local mechanical anisotropy regulates ovarian cancer invasion

doi: 10.1038/s41467-025-62296-7

Figure Lengend Snippet: a Schematic representation of the organo-hydrogels (OHGs) with varying microdroplet diameter and volume fraction. b Storage modulus of collagen-based OHG with distinct microdroplet size ( n = 4 gels; average ± s.e.m.). The dashed line shows the predicted trend. c Normalised stress-relaxation curves of collagen-based OHG with distinct microdroplet size ( n = 4 gels). d BODIPY (green) and F-actin (red) and nuclei (blue) staining of OVCAR8 cells after 7 d in collagen-based OHG of 70 or 25 μm diameter microdroplets (representative images from n = 13 spheroids). Arrowheads indicate cells at the invasive front. e Quantification of relative OVCAR8 spheroid area in collagen-based OHG of distinct emulsion percentage of volume fraction and microdroplet diameter after 7 d in culture ( n = 13 spheroids). f BODIPY (green) and mRFP (red) staining of mRFP-OVCAR8 cells after 7 d invasion into adipose peritoneal tissue explants (representative images from n = 3 explants per donor). g Correlation between patient average adipocyte diameter and invaded OVCAR8 cells after 7 d, at 42 μm tissue depth, relative to number of cells at the tissue surface ( n = 6 donors; average ± s.e.m.). The dashed line shows the predicted trend. h Quantification of interdroplet distance in OHG ( n = 100 microdroplets). i Quantification of the percentage of invasive front OVCAR8 cells in contact with microdroplets in OHG ( n = 121 cells in 3 gels). j Quantification of percentage of Ki67 + cells, immunofluorescence signal intensity of cytoplasmic pFAK and pMLC relative to collagen average, and nuclear:cytoplasmic ratio of YAP/TAZ in OVCAR8 cells embedded in OHG of 70 or 25 μm diameter microdroplets for 24 h ( n = 55 cells). k F-actin (green) and DAPI (blue) staining of CAOV3 spheroids embedded for 7 d in collagen-based 25 μm microdroplet OHG in the presence or absence of TGFβ. Arrowheads indicate invading cells (representative images from n = 6 spheroids). l Quantification of CAOV3 spheroid area relative to non-TGFβ-treated control after 7 d in culture ( n = 6 spheroids). m Schematic illustration of the cell force-dependent invasion of adipose tissue enabled by the anisotropic mechanics of OHGs and the generation of migration tracks at the ECM-adipocyte/microdroplet interface. Arrows indicate the direction of the force. For the data in ( h , i , j and l ), a two-sided unpaired t test was performed. Coefficient of determination and Pearson correlation (two-tailed test) were performed in ( g ) to determine the relationship between tissue adipocyte diameter and OVCAR8 invasion. Error bars in ( b ) represent the s.e.m. Scale bars, 50 μm (bottom panels in d , k ), 100 μm ( f ), 200 μm (top panels in d , k ). Source data are provided as a Source Data file.

Techniques: Staining, Emulsion, Immunofluorescence, Control, Migration, Two Tailed Test